DMEM/F12(1:1)+10%FBS+1% ITS( GIBCO 41400045 )+50 nM Hydrocortisone+1 ng/ml EGF+0.01 mM ethanolamine+0.01 mM phosphorylethanolamine+100 pM triiodothyronine+0.5% (w/v) bovine serum albumin+0.5 mM sodium pyruvate+2mM L-glutamine We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium:
90%FBS+10%DMSO
Comments:
For more information, please contact Cobioer (4008-750-250).
